Genetic analysis of a soybean genetic pool using ISSR marker: effect of gamma radiation on genetic variability.

摘要

Various studies showed that the genetic diversity in soybean (Glycine max L.) germplasm is limited. There is insufficient data on molecular analysis of soybean collections from Africa. The main objectives of the present study were (1) to analyze the genetic diversity and relationships among soybean accessions identified in the DR-Congo gene pool and (2) to determine the effect of gamma radiation on genetic variability. Genomic DNA from several cowpea accessions were analyzed using Inter-simple Sequence Repeat (ISSR) system. The level of polymorphic loci among the soybean varieties was high, varying from 70 to 90% based on ISSR primers used. The soybean varieties analyzed were genetically closely related with several accessions exhibiting similar ISSR amplification profiles. The genetic distance among the soybean accessions varied from 0.00 to 0.46. Some accessions from the International Institute of Tropical Agriculture (ITTA) revealed identical ISSR amplification profile. Seed treatment with gamma-rays at 0.2 KGy (20 Krad) increases the level of polymorphism in progenies by 10%. Low genetic diversity observed within varieties was increased with gamma-ray treatment at 0.1 KGy (10 Krad) and 0.2 KGy (20 Krad) dose.