Organogenesis of Bambara Groundnut (Vigna subterranea (L.) Verde.) through in Vitro Culture from Nodal Segments

摘要

This study was designed to evaluate the effects of plant growth regulators - auxin (NAA) and cytokinin (BA) - on the regeneration potential of two accessions of bambara groundnut 'TVSul834' (Nigeria) and 'TVSu255' (Niger) through in vitro culture. Nodal vine segments from 28-days-old plantlets derived from the embryonic axis were used as explants for nodal culture. The explants were cultured on half-strength MS basal salts supplemented with different concentrations and combinations of cytokinin and auxins for primary shoot proliferation. BA at 0.45 mg/1 in combination with three different concentrations of NAA, namely 0 mg/1 (M4), 0.15 mg/1 (M2) and 0.25 mg/1 (M3) were used for nodal culture. The control medium Ml, had no plant growth regulators added. The shoots, leaves, and whole plant regeneration were evaluated at 4 weeks by means of percentage of explants producing shoot, callus and rooting per explant. The best shoot proliferation of nodal cuttings of 'TVSul834' was observed in M3 medium containing half-strength MS, 0.45 mg/1 BA, 0.25 mg/1 NAA and 100 mg/1 myo-inositol which produced multiple shoots with the longest shoots and most leaves compared to 'TVSu255'. In Ml medium, the control, taller plantlets formed than at other concentrations and was the only medium that induced rooting. There was a genotype-dependent effect (P < 0.05) on shoot proliferation, shoot length and number of leaves in the two accessions.