Non-invasive detecting human tissue hemoglobin concentrations using near-infrared spectroscopy with in vivo calibration

摘要

Human tissue oxygenation status can be completely indicated by its hemoglobin concentrations. To detect them non-invasively using continuous-wave near infrared spectroscopy (NIRS), the 'in vivo calibration' algorithm, which was comparing tissue absorption before and after the change of tissue oxygenation, was adopted to obtain its reduced scattering coefficient. The hemoglobin concentrations of forearm muscle of four adults during occlusion, and those of brain cortex of three newborns during oxygen inhalation were detected. For the four adults, the μ_s~' of the forearm muscle were 6.01～7.87 cm~(-1) under 758 nm, and its total hemoglobin concentration (C_(tHb)) was 37.5 ± 3.1μM at the beginning of occlusion. For the three newborns, the cerebral μ_s~' were 5.46～8.42 cm~(-1) under 758 nm, and C_(tHb) was 50.1 ± 6.1μM at the beginning of oxygen inhalation. All the results were consistent with most recent reports. In summary, the in vivo calibration algorithm is effective to detect human tissue hemoglobin concentrations.