Atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) on a quadrupole ion trap mass spectrometer

摘要

A new atmospheric pressure ionization technique, atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI), was recently introduced by Laiko et al. [U.S. Patent 5,965,884; Abstracts of the 4th International Symposium on Mass Spectrometry in the Health and Life Sciences, San Francisco, CA, 25-29 August 1998; Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry, Proceedings of the 47th ASMS Conference on Mass Spectrometry and Allied Topics, Dallas, TX, 1999; Anal. Chem. 72 (2000) 652]. This source has been coupled to an orthogonal time-of-flight mass spectrometer [U.S. Patent 5,965,884; Abstracts of the 4th International Symposium on Mass Spectrometry in the Health and Life Sciences, San Francisco, CA, 25-29 August 1998; Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry, Proceedings of the 47th ASMS Conference on Mass Spectrometry and Allied Topics, Dallas, TX, 1999; Anal. Chem. 72 (2000) 652] and to an ion trap mass spectrometer (ITMS) [Anal. Chem. 72 (2000) 5239; Atmospheric Pressure MALDI/Ion Trap Mass Spectrometry, Proceedings of the 48th ASMS Conference on Mass Spectrometry and Allied Topics, Long Beach, CA, June 2000: An Atmospheric Pressure MALDI Probe for Use with ESI Source Interfaces, Proceedings of the 48th Asms Conference on Mass Spectrometry and Allied Topics, Long Beach, CA, June 2000; Atmospheric Pressure Matrix-Assisted Laser Desorption Ionization, Proceedings of the 48th ASMS Conference on Mass Spectrometry and Allied Topics, Long Beach, CA, June 2000]. Here, we present the current status of the work involving the development of an AP MALDI source for an ITMS. In addition, we present recent work from our own laboratory to demonstrate the utility of this novel configuration.