Modulation of energy metabolic enzyme expression in N-nitrosodiethylamine- mediated hepatocarcinogenesis by Chinese herbs, Huqi San

摘要

Aim: To elucidate the anticancer mechanism of Huqi San by assessing the expression of G-6-Pase, SDH, ATPaseand AFP in N-nitrosodiethylamine-mediated hepatocarcinogenesis in rats. Methods: A Solt-Farber two-step test model of hepatocarcino genesis was established by diethylnitrosamine (DEN) and2-acetylaminofluorene (AAF) in rats to investigate the modifying effects of expression of 6-glucosephosphatase (G-6-Pase),succinodehydrogenase (SDH), adenosine triphosphatase (ATPase) in N-nitrosodiethylamine-mediated hepatocarcinogenesis.Hu Qisan compounded by eight medicinal herbs was prepared in glycoprival granules with wich 0.38 g crude herbs/mL solutionwas prepared for administration. ry-Glutamy-transpeptidase (-y-GT), G-6-Pase, SDH and ATPase were immunohistochemicallydetermined. The activity of alpha-fetoglobulin (AFP) in the livers was measured with Immunofluorescence.Results: Huqi San treated rats showed significant decrease in areas of -y-GT positive foci (P < 0.001). On the other hand,the expression of G-6-Pase, SDH and ATPase has obviously altered in Huqi San treated group. The activity of AFP alsosignificantly decreased after the treatment with Huqi San (8 g/kg body weight or 4 g/kg body weight) or total alkali of mistletoe(0.12 g/kg body weight).Conclusions: Huqi San can obviously increase these activities of G-6-Pase, SDH and ATPase, and at the same timesignificantly decrease the expression of ry-GT and AFP. Therefore, it can obstruct or inhibit the rat's liver preneolastic lesioninduced by DEN.