Portuguese winemaking residues as a potential source of natural anti-adenoviral agents.

摘要

To date there are no licensed systemic or topical treatments in Europe or the USA for adenovirus infections. In this study, the effects of a polyphenol-based grape extract (NE) obtained from Portuguese white winemaking by-products, and resveratrol in pure form, were evaluated upon adenovirus type 5 infection. Recombinant adenovirus vectors (Ad-5) and a human-derived cell line (293) were used as models. NE and resveratrol did not induce cell cytotoxicity or direct virucidal activity, however, they reduced 4.5 and 6.5 log (TCID50/ml) of total infectious Ad-5 production, respectively. The capacity of Ad-5 replication upon removal of NE and resveratrol after 24 h post infection was also evaluated. In contrast to resveratrol, the highest evaluated NE concn. irreversibly inhibited Ad-5 replication. It is suggested that results may provide useful information for the use of NE and resveratrol as potential sources of natural antiviral agents against Ad-5 infection.