Screening and comparing tocopherols in the rapeseed (Brassica napus L.) and olive (Olea europaea L.) varieties using high-performance liquid chromatography.

摘要

This study sought to investigate some new rapeseed varieties and olive genotypes grown in northwest Turkey for tocopherol fractions and contents. For rapeseed, the data were collected in 2 growing seasons (2004-2005 and 2005-2006) from a field experiment with 19 new rapeseed varieties. For olives, virgin olive oils produced from 21 different varieties were examined in the 2004-2005 and 2005-2006 growing seasons. Separation and identification of tocopherols and the analysis of their contents were successfully achieved by HPLC. In rapeseeds, gamma-tocopherol (44.2-118.9 mg/kg) was the major fraction, followed by alpha-tocopherol (19.3-68.50 mg/kg) and delta-tocopherol (0-2.60 mg/kg). In olive varieties, alpha-tocopherol contents were 52.000-194.750 mg/kg and those of gamma-tocopherol were 0-39.75 mg/kg. Total tocopherol contents ranged between 83.900 and 173.800 mg/kg for rapeseed and between 52.100 and 213.075 mg/kg for olive genotypes. Results suggest that tocopherol contents and composition vary between rapeseed and olive genotypes.