Identification and quantitative analysis of kojic acid and arbutine in skin-whitening cosmetics

摘要

After testing several chromatographic systems described in the literature, the optimal operating con- ditions were retained to develop a simple and rapid method grouping TLC and HPLC/UV for separation, identification and dosage of kojic acid (CAS RN 501- 30-4) or arbutine ( CAS RN 497- 76- 7) in skin-whiten- ing cosmetic products. The screening is carried out by TLC on cellulose plate and by TLC on silicagel plate with UV indicator and polar mobile solvent. Regard- ing identification and quantitative determination by HPLC/UV; the best results were obtained by direct phase chromatography, using a polar stationary phase greffed with diol groups and a polar buffered mobile phase at PH 2,5. lntralaboratory prevalidation tests were carried out on the HPLC method: detection limit, linearity and intralaboratory repeatability of standard curves and repeatability of samples quantitative determination.