Production of Canola Protein Materials by Alkaline Extraction, Precipitation, and Membrane Processing

摘要

ABSTRACTA process designed for the characteristics of rapeseed and canola proteins was developed, consisting of extraction of oil‐free meal at pH 10.5–12.5, isoelectric precipitation to recover proteins and ultrafiltration followed by diafiltration to concentrate and purify the remaining acid‐soluble proteins. These steps complement one another to yield three products with excellent protein recovery. Isoelectric and soluble protein isolates containing 87–104 protein (N × 6.25) and a meal residue were obtained from canola meal. All five types of fractions were free of glucosinolates. The two isolates were low in phytate, light in color, and bland in taste. The isolate yield depended on the starting meal. The process is simple and has good potential for commercial app