ABSTRACTSubcellular distribution of fatty acids in the membranes and cytoplasm of muscle and adipose tissues from USDA Select beef carcasses was determined. Cholesterol in subcellular fractions of intramuscular adipose tissue also was quantified. There was a greater percentage of saturated fatty acids in cytoplasmic, than in membrane, fractions of muscle. With the exception of intramuscular adipose tissue, monounsaturated fatty acids constituted a greater percentage of the total lipid in the cytoplasm than in membranes. There was a greater percentage of polyunsaturated fatty acids in the membrane, than in the cytoplasmic, fraction. Cholesterol averaged 118 mg per 100 g intramuscular adipose tissue with 54 in the cytoplasm and 46 in the membranes.
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