Effect of Different Periods of Storage and Heating Temperatures of Total Mixed Rations Containing Fibrolytic Enzymes on Enzyme Activity and In Vitro digestibility

摘要

Effect of different periods of storage and temperatures of total mixed rations (TMR) containing exogenous fibrolytic enzymes (EFE) on enzyme activity and in vitro digestibility was studied. Cellulase (4026 mu M glucose/g/h) and xylanase (7825 mu M xylose/g/min) were mixed in equal proportion (w/w) and added at 1.5 and 3.0 g/ kg DM of TMR to the concentrate portion of TMR. The concentrates were subjected to heating at 40,50,60 and 80°C in a hot air oven for 2,5 and 10 minutes in first experiment andstored in airtight polythene bags at room temperature (30-34°C) for 0, 7, 15, 30, 45 and 60 days in another experiment. Thereafter, concentrates containing EFE were mixed with wheat straw and green fodder (sorghum) in 25:60:15 (TMR I), 40:45:15 (TMR II)and 60:25:15 (TMR III) ratios on DM basis and IVDMD, IVNDFD and IVADFD were estimated at 48 hours post incubation. There was no adverse effect of heating on cellulase and xylanase activities, irrespective of temperature and heating time as well as storage up to 60 days. Similarly, there was no effect of heating concentrate containing 1.5 g and 3.0 g EFE/kg DM to 40,50,60 and 80°C for 2, 5 and 10 minutes or storing up to 60 days on percent IVDMD, IVNDFD and IVADFD of the three TMRs containing these concentrates. It was concluded that there was no adverse effect of storage (up to 60 days) and heating (up to 80°C) of total mixed rations containing fibrolytic enzymes on cellulase and xylanase activities and in vitro fiber digestibility.