Chromosome preparation for high resolution G-banding in crossbred cattle using ethidium bromide treatment.

摘要

Exposure of cattle lymphocytes in late G2 phase of the cell cycle to ethidium bromide (EB) resulted in accumulation of cells in prophase and pro-metaphase stages, and also inhibited chromosome condensation. A simple method for obtaining high resolution GTG banding patterns on elongated chromosomes in peripheral lymphocyte culture of cattle exposed to 10 鎔/ml EB without colchicine is described. High resolution GTG banding was obtained by mild trypsin treatment followed by staining with Giemsa.