A Method for the Analysis of ATP Metabolites in Beef Skeletal Muscle by HPLC

摘要

ABSTRACTA method to determine inosine, hypoxanthine, NAD, AMP, IMP, IDP, ADP, adenylosuccinic acid, ITP and ATP contents in postmortem muscle by HPLC has been developed. The method consists of an homogenization of the muscle in 0.5M HClO4or 10 TCA, extraction of the compound with 0.5M tri‐n‐octylamine/Freon 113 and single injection chromatography on a Whatman Partisil‐10/25 SAX c