A COMPARATIVE STUDY ON THREE SEROLOGICAL TESTS USED TO DETECT CAPRINE BRUCELLOSIS

摘要

Serum samples collected from 181 goats were tested by employing the rose bengal test (RBT), standard tube agglutination test (STAT) and indirect enzyme linked immunosorbent assay (i-ELISA). While the seropositivity in goats by i-ELISA was 24.86 per cent, the RBT was able to detect 17.68 per cent. The STAT, however detected only 16.02 per cent. The i-ELISA was found to be sensitive in detecting anti-Brucella melitensis antibodies in goats. The RBT and STAT were compared with the standard test viz. i-ELISA. The study revealed that the sensitivity and specificity of RBT with i-ELISA were found to be 55.55 per cent and 94.85 per cent, respectively. The sensitivity and specificity of STAT with i-ELISA were found to be 57.78 per cent and 97.79 per cent, respectively.