SUMMARYIn this study, the mode of action of vitamin K5was investigated withSaccharomyces cerevisiaethe test organism.Cysteine hydrochloride antagonized the anti‐fungal action of vitamin K, suggesting that the vitamin has an affinity for sulfhydryl groups, which are necessary for the activity of certain enzymes. The sulfhydryl groups of yeast cells were reduced quantitatively when they were exposed to vitamin K5.Furthermore, studies showed that dehydrogenase was inactivated by vitamin K
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