NUCLEOTIDE SEQUENCE POLYMORPHISM WITHIN EXON 8 OF HEAT SHOCK PROTEIN (HSP) 90AA1 GENE AND ITS ASSOCIATION WITH MILK PRODUCTION TRAITS IN DEONI COWS

摘要

A group of proteins known as Heat Shock Proteins (HSPs) are synthesized during heat stress. HSP genes have been reported to be associated with heat tolerance and production performance in cattle. PCR-SSCP technique and direct sequencing was used to identify novel SNPs in Exon 8 of HSP90AA1 gene in Deoni cows. In Exon 8, three unique SSCP patterns corresponding to TT, GG and TG genotype with frequencies of 0.250, 0.639 and 0.111, respectively were observed. The sequence analysis of Exon 8 revealed T -> G transversion at position 3650 of HSPAA1 gene (GenBank accession number NC-007319 as reference sequence). The observed polymorphism (T -> G) at position 3650 results in substitution of an amino acid from Phenylalanine to Leucine. The cows having TG genotype had significantly higher lactationMilk Yield (Kg) as compared to cows with TT and GG genotypes (P <= 0.01). There was no difference in Lactation Length (days) in cows with different genotypes.