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Evaluation of micromineral status and ovarian hormonal profile and their interrelation in normal cyclings and repeat breeding cows

机译:正常骑行和重复繁殖母牛的微量矿物质状态和卵巢激素谱及其相互关系的评估

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Blood samples from 12 normal cycling and 12 repeat breeding crossbred cows were collected aseptically on different phages of oestrous cycle to elucidate the possible relationship between the trace elements and hormone profile of ovary in normal cycling and repeat breeding cross bred cows:Different trace elements namely Fe, Cu, Zn and Mn were estimated using Atomic Absorption Spectrophometer (AAS) and serum oestradiol 17-13 and progesterone were estimated using coat A -count RIA kit. Fe, Cu and Mn concentration was observed apparently high and Zn concentration was significantly (P<0.05) high in each phase of collection in normal cycling cows from repeat breeder except mid luteal phase. Oestradiol 1743 concentration was significantly (P<0.01) high in normal cycling cows in oestrous and early luteal phase, while no significant variation was observed for progesterone concentration between the same phase of the two groups. A positive significant (P<0.01) correlation was observed between Fe-Zn and Cu-Zn (P<0.05) and oestradiol 1743 (P<0.05) in normal cycling cows. In repeat breeding cows Fe and oestradiol 1743 and Mn and progresterone was positively significant (P<0.01).
机译:在不同周期的噬菌体上无菌采集了12头正常自行车和12头重复繁殖杂交奶牛的血样,以阐明正常循环和重复繁殖杂交奶牛卵巢微量元素与激素谱之间的可能关系:不同的微量元素即Fe ,使用原子吸收光谱仪(AAS)估算铜,锌和锰,并使用A计数RIA试剂盒估算血清雌二醇17-13和孕酮。除黄体中期外,在重复育种的正常循环奶牛的每个收集阶段中,铁,铜和锰的浓度均明显较高,而锌的浓度均显着(P <0.05)高。在正常雌性黄牛和黄体早期的周期奶牛中,雌二醇1743的浓度显着较高(P <0.01),而在两组的同一相之间,孕酮的浓度没有显着变化。在正常骑自行车的奶牛中,铁锌和铜锌(P <0.05)与雌二醇1743(P <0.05)之间存在正相关(P <0.01)。在重复繁殖的母牛中,Fe和雌二醇1743以及Mn和Progresterone呈显着正相关(P <0.01)。

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