PEANUT ROOT EXTRACTS AND RESVERATROL INHIBIT LIPOPOLYSACCHARIDE-INDUCED INFLAMMATION BY SUPPRESSION OF MAPKS SIGNALING PATHWAYS IN BV-2 CELLS

摘要

Peanut Root (PR), in the field of agricultural waste, contains resveratrol and bear potent antioxidative activity. The effect of the PR extract and resveratrol on lipopolysaccharide (LPS) induced inflammation of BV-2 microglial cells and acute liver injury in Sprague-Dawley (SD) rats was investigated. The results showed that the PR extract and resveratrol reduced LPS-induced nitric oxide (NO), interleukin (IL)-l, IL-6, reactive oxygen species (ROS), and prostaglandin E_2 (PGE_2) production in BV-2 cells. The PR extract and resveratrol significantly decreased serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in LPS-treated rats. Furthermore, the water extract, with exception of the ethanol extract, of the PR extract dose-dependently inhibit LPS-induced mitogen-activated protein kinases (MAPK), and slightly inhibit cyclooxygenase-2 (COX-2) expression in BV-2 cells. Taken together, these results indicated that the protective mechanism of PR extract involved an antioxidant effect and inhibition of MAP kinases and COX-2 expressions in LPS-induced inflammation of BV-2 cells.