Studies on Production of 3-Hydroxypropionaldehyde from Glucose by a Novel Enterococcus Dispar: Optimization of Medium Components by Statistical Experimental Design

摘要

Production of 3-Hydroxypropionaldehyde (3-HPA) by a novel strain Enterococcus dispar was studied for the first time from glucose. 3-HPA is an intermediate compound in the metabolic conversion of glucose to 1,3-propanediol, which must be trapped by semicarbazide to arrest the pathway to get the required 3-HPA. To maximize the 3-HPA concentration, different factors such as, age of inoculum, rpm, pH, glucose, semicarbazide hydrochloride, K2HPO4 and inoculum level were selected based on the single variable optimization studies. These factors were considered at three levels (18 experiments) in the experimental design of Taguchi. It was observed from Taguchi experimentation that the pH of the medium was found to be the most significant factor among all selected parameters. The combination of factors such as age of inoculum and glucose resulted in higher interacting severity index of 75.12 %, suggesting their interactive role in the regulation of 3-HPA production in this novel microbial species. Analysisof variance (ANOVA) was performed on the obtained results. The yield of 3-HPA was increased irom 6 mg/ml of conventional method of optimization to 9.55 mg/ml of Taguchi approach, which represents 37.17% enhancement in the production.