Changes in expression of CD45R during the development of Th1 and Th2 cell lines

摘要

AbstractPrevious studies using anti‐CD45R monoclonal antibodies (mAb) have shown that normal CD4+T cells can be separated into virgin and memory cells basedon their level of expression of CD45R. CD45Rhi(virgin)T cells secrete interleukin (IL)‐2 whereas CD45Rlo(memory) T cells secrete both IL‐2 and IL‐4. In contrast to these results, studies using cultured T cell lines haveshown that IL‐2‐secreting T helper cell type 1 (Th1) cells are CD45Rloand IL‐4‐secreting Th2 cells are CD45Rhi. To resolve this discrepancy, and to determine how the Th1 and Th2 cell lines relate to memory CD45Rloand virgin CD45RhiCD4+T cells, we have isolated CD45Rlocells from alloantigen‐primed mice and developed allospecific Th1 and Th2 cell lines. The lymphokinessecreted by these lines were then evaluated. Our results show that as CD45RloT cells develop intoin vitrocell lines which secrete IL‐4, they become CD45Rhi. In contrast, CD45Rlocells that develop into lines which secrete IL‐2 maintain their CD45Rlophenotype. Thus, although both Th1 and Th2 cells arise initially from CD45Rlocells, Th2 cells become CD45Rhiduring prolongedin vitroculture