STUDY ON ENDOPHYTIC MYCOBACTERIUM SECONDARY METABOLITES AND ANTI - TUMOR ACTIVITY OF CERVICAL CANCER IN THREE STRAINS OF CRUDE EXTRACTS

摘要

To study the anti-cervical cancer activity of three secondary metabolites of endophytic fungi from Ginkgo biloba. The species of three strains were determined by the combination of colony morphology and 18 sRNA sequencing. Then the anti-cervical cancer activity of three strains of crude extracts was tested by MTT assay. Finally, the inhibitory effect Secondary metabolites of more than 50 of the isolates were isolated and the compound monomers with anti-tumor activity were screened. Colony morphology and 18 sRNA sequencing showed that strains J-1 and J-2 belong to the genus Fusarium and J-3 may belong to the genus Fusarium, but further validation is needed. MTT assay showed that the growth inhibition rates of crude extracts of strain J-1 and J-3 on Hela cells were 59.6 and 59.1, respectively. Semi-preparative HPLC combined with MTT method to obtain four compounds with anti-cervical cancer activity of the monomer, the need for further structural identification. Two ginkgo endophytic fungi with strong anti-cervical cancer activity were obtained, indicating that endophytic fungi of Ginkgo biloba as a potential source of anti-tumor drugs deserve attention.