Use of Glycosyl Dithiocarbamates: Small Molecule 'Turn-on' Fluorescent Probe for Carbohydrate Binding Proteins

摘要

A glycosyl dithiocarbamate derivative is developed as a "turn on" fluorescent sensor for lectins. The probe is prepared through a three-component reaction in good yield and fully characterized by analytical experiments. Upon interaction with lectin ConA, the virtually non-fluorescent probe has shown "turn on" sensing with absolute specificity in terms of the carbohydrate used, mannose in this particular case. The probe with galactose remained inactive with ConA. The simple and general strategy for the synthesis of this particular probe offers the scope for multiple probes by altering the carbohydrate moiety and thus, opens up the avenue to target various lectin sensing.