AN IMPROVED SEMIAUTOMATED ENZYMATIC ASSAY OF LYSINE IFU FOODSTUFFS

摘要

ABSTRACTAn improved semiautomated enzymatic procedure employing L‐ly‐sine decarboxylase for the analysis of lysine is described. Carbon dioxide formed in the reaction mixture is separated by a gas dialyzel and measured calorimetrically by the phenolphthalein method. Certain vitamins, amino acids, and metal ions, when added to lysine standard solution and analyzed by the present procedure, caused a decrease in both sensitivity and reproducibility. The procedure developed is capable of analyzing 30 samples per hour. The mean recovery of added lysine was 94