Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx)

摘要

Phospholipid hydroperoxide glutathione peroxidase (PHGPx), as a ubiquitous antioxidant enzyme in the glutathione peroxidases (GPx) family, plays multiple roles in organisms. However, there is very little information on PHGPx in goats (Capra hircus). In this study, a full-length cDNA was cloned and characterized from Taihang black goat testes. The 844 bp cDNA contains an open reading frame (ORF) of 597 bp. The goat PHGPx nucleotide sequence contains a selenocysteine (sec) codon TGA 244-246, two potential start codons ATG 20-22 and ATG 108-110, a polyadenylation signal AATAAA 813-818 and selenocysteine insertion sequence (SECIS) motif AUGA 688-691, UGA 729-731 and AAA 703-705. As a selenoprotein, the active-site motifs and GPx family signature motifs LAFPCNQF 101-108 and WNFEK 165-170 were also found. The order of PHGPx mRNA expression levels was: testes heart brain epididymis kidney liver lung spleen muscle. Real-time PCR and immunohistochemistry results revealed similar expression differences in different age testes, with high expression levels during adolescence. Immunofluorescence results suggested that PHGPx mainly expressed in Leydig cells and spermatids in mature goat testes.