DETERMINATION OF ACETALDEHYDE IN APPLE TISSUE BY 2,4‐DINITROPHENYLHYDRAZONE FORMATION

摘要

ABSTRACTSampling involved maceration of apple tissue in acidified 2,4‐dinitrophenylhydrazine, followed by extraction of the carboxyl‐2,4‐DNP‐hydrazones with benzene. Acetaldehyde‐2,4‐DNP‐hydrazone was separated and purified by TLC and quantitatively determined by UV spectroscopy at 354 nm. The identity of acetaldehyde‐2,4‐DNP‐hydrazone was confirmed by mass spectroscopy and gas‐