Characteristics of Increased Serum Amyloid A (SAA) and alpha?Acid Glycoprotein (AAG) Concentrations in Cats Subjected to Experimental Surgical Treatments or Inoculated with Bordetella bronchiseptica

摘要

The concentrations of serum amyloid A (SAA) and alpha l?acid glycoprotein (AAG) in the serum of 11 healthy cats subjected to oophorohysterectomy (5 females) and gastrotomy (3 males, 3 females) were measured by turbidimetric immunoassay (TIA) for humanSAA (human SAA-TIA) and single radial immunodiffusion (SRID) kits for feline AAG, respectively. Six healthy cats (3 males, 3 females) were inoculated intrabronchially with 2 X 109 live, aviru-lent cells of Bordetella bronchiseptica as a model of transient bronchopneumonia in order to investigate serum concentrations of SAA and AAG, as well as white blood cell (WBC) counts and antibody responses to B. bronchiseptica in sera by indirect fluorescence antibody technique (IFA). Peak concentrations of SAA after oophorohysterectomy and gastrotomy were 4.8 to 26.8 times and 11.5 to 23.4 times higher than pre-treatment concentrations, respectively, while those of AAG were 1.7 to 4.1 times and 1.8 to 2.6 times higher than pre-treatment concentrations, respectively. The ratio of pre-treatment and peak serum concentrations of SAA and AAG in cats inoculated with B. bronchiseptica ranged from 0.4 to 20.1 and 1.3 to 2.9, respectively. In these experiments, peak serum concentrations of SAA and AAG were observed at 1or 2 days after surgical treatments and inoculation with B. bronchiseptica. SAA was not detected at 5 days after these treatments, except in 1 cat after oophorohysterectomy. Strong individual variations in SAA and AAG concentrations were observed in thepresent study.