SPECTROPHOTOMETRIC DETERMINATION OF THEOBROMINE IN T. cacao, C. acuminata AND C. arabica

摘要

ABSTRACTA number of different techniques are reported in the literature for the extraction and purification of theobromine as well as quantitative methods following purification. This research note reports a procedure offering considerable time saving for the determination of theobromine inT. cacao, C acuminataandC arabicaThe procedure incorporates the Wadsworth extraction technique, gradient elution partition chromatography and quantitative estimation of theobromine spectrophotometrically.