Rational Design of Semi-Synthetic Protein Complexes with the Defined Oligomeric State

摘要

abstract_textpProtein oligomers are ubiquitous in nature and play an essential role in various biological processes. Recently, there is an enormous interest to custom-make synthetic protein oligomers through the bottom-up approach. In this regard, genetic methods have made tremendous progress in the last decade. In comparison, only few chemical methods currently exist for this purpose. Herein, we report a modular synthetic strategy for the design of facially amphiphilic globular protein-synthetic peptide conjugates. The self-assembly of these bioconjugates is driven by strong hydrophobic interaction of the peptide domain. The size, molecular mass and oligomeric state of semi-synthetic protein complexes strongly depend on size and surface charge of a globular protein as well as the length of the hydrophobic peptide domain. A systematic structure-property relationship study of these semi-synthetic proteins should shed more light on the understanding of various non-covalent forces, which governs the oligomerization processes of naturally occurring proteins./p/abstract_text