UV Damage and Repair in the Domain of the Humanc–mycOncogene

摘要

ABSTRACTWe have used a modification of the Southern hybridization method to analyze the removal of UV-induced pyrimidine cyclobutane dimers from the domain of the c-myconcogene. The study was performed in human COLO320HSR cells, which exhibit a 30- to 40-fold amplification of c-mycthat is maintained in a marker chromosome as a homogeneously staining region. Intron 2 and the region upstream from the gene showed better dimer removal than intron 1 or the region downstream from the c-mycgene. Regions showing less repair coincide with regions that are hotspots for mutations and chromosome translocations. Therefore, it is proposed that the inefficiency of DNA repair may play an important role in the origin of c-mycrearrangements.