Effects of ginsenosides on carbachol-stimulated formation of inositol phosphates in rat cortical cell cultures.

摘要

We examined the effect of ginseng total saponins (GTS) on phosphoinositide metabolism stimulated by activation of muscarinic receptor using rat cortical cultures. Carbachol stimulated formation of 3Hinositol phosphates (3HInsPs) by 3.3-fold over basal level in 3Hinositol-prelabeled cells. Pretreatment of GTS inhibited formation of 3HInsPs evoked by carbachol by 70-90. Addition of GTS alone had no effect on the basal formation of 3HInsPs. The inhibitory effect of the GTS on carbachol-stimulated formation of 3HInsPs was dose- and time-dependent. IC50 was 6.0 +/- 2.8 microg/ml. We also examined the effect of GTS on 3HInsP1, 3HInsP2, or 3HInsP3 formation evoked by carbachol. Although GTS had no effect on the basal 3HInsP1, 3HInsP2, or 3HInsP3 formation, pretreatment of GTS inhibited 3HInsP1, 3HInsP2, or 3HInsP3 formation evoked by carbachol, respectively. Addition of individual ginsenosides such as ginsenoside Rb1, Rc, Rd, Re, or Rg2 had no effect on the basal formation of 3HInsPs, whereas pretreatment of ginsenoside Rb2, Rc, Rd, Re, Rf, Rg1 or Rg2 inhibited formation of 3HInsPs evoked by carbachol by 79-89. The results suggest that the inhibitory effect of GTS and its individual ginsenosides on carbachol-stimulated formation of 3HInsPs in cortical neurons could be one pharmacological action of Panax ginseng.