A study was carried out to determine how14C applied as14CO2to a localized area of the leaf for 5ensp;min moved to the rest of the leaf during the treatment period. It was concluded that14CO2diffuses from the application area to the rest of the leaf where it is fixed photosynthetically in the position detected by whole leaf autoradiography. These conclusions were deduced from experiments on darkening a portion of the leaf, comparing the radioactive compounds in treated and untreated areas of the leaf, and by using variegatedPelargoniumleaves. Using the darkening technique,14CO2movement in the stem of soybean was not detected for 15ensp;min after treatment of a primary leaf with14CO2in the light.
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