RESEARCHES ON IN VI T R0 GERMINATION AND SEEDLIN G DEVELOPMENT OF MEDICINAL ENDEMIC NEPET A VISCIDA BOISS. BELONGING LAMIACEAE FAMILY

摘要

The present study examins in vitro germination and seedling development of Nepeta viscida Boiss., an endemic medicinal plant. At the first step, effects of various in vitro media, gibberellic acid, illumination and pH on germination were investigated. The best germination rate of N. viscida seeds was obtained on DW medium with 2 mg/l GA3 (75). Giberellins were played important role of seed germination. Light has a positive effect on germination and required for the N. viscida seeds. At the second step, determination of appropriate medium for in vitro seedlings development was investigated. Seedlings obtained from germinated seeds were transferred to MS (Murashige and Skoog, 1962), B5 and WH (White) media. After 8 weeks from culture initiation, development of seedlings (maximum shoot length, shoot number, leaf number, root length and root number) were evaluated. MS was observed as the most suitable medium for seedling development. The nutrient medium type has caused changes in the level of photosynthetic pigment. Chlorophyll a and chlorophyll b levels of seedlings grown on the WH medium were decreased by 78 and 80, respectively, compared to seedlings grown on the MS medium. This report represents an efficient method for the ^n vitro propagation of Nepeta viscida Boiss.