PURIFICATION OF PORCINE LEUKOCYTE LYSOSOMAL HYDROLASES

摘要

ABSTRACTLeukocytes were separated from porcine blood and their cellular components fractionated either by differential centrifugation or by n‐butyl alcohol extraction. Acid phosphatase, aryl sulfatases, β‐glucuronidase and cathepsin D were purified by DEAE‐cellulose and Sephadex column chromatography and by polyacrylamide gel electrophoresis. Histochemical reactions were used to identify enzymes in the latter pro