The body metabolism and the mineral status of the cattle and buffaloes can be improved by supplementing the organic mineral complexes as additive feed. In the present work, the concentration of cobalt and alkaline phosphatase (ALP) in blood-serum of one hundred and eighty buffaloes (lactating, non-lac-tating and pregnant) was estimated at livestock farms of three districts of Punjab. The milk yield of pregnant and lactating animals was also determined. The cobalt level was found to be different in soil, forage and water of three districts of Punjab. The animals were placed into healthy group (HG), affected untreated group (UG) and treated group (TG) after comparing the serum levels of Co and ALP with their respective critical values. The TG group of animals was provided with normal diet along with the synthesized cobalt methionine complex after FT-IR characterization while other animals of all classes were provided normal diet only. After supplementing cobalt methionine complex, the serum level of cobalt increased from 60.02± 1.7 to 72.2±4.5, 52.3±2.5 to 63.4±2.03 and 56.1±0.9 to 67.1±1.4 ^g/dl in lactating, non-lactating and pregnant animals respectively and the serum level of ALP highly decreased from 173.6±4.2 to 158.5±0.9 U/l in pregnant animals and the milk yield increased from 6.2±0.22 to 7.09±0.3 and 5.4± 0.1 to 6.5± 0.14 Lt/day in lactating and pregnant animals respectively of TG groups at district Bhakkar.
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