Simultaneous analysis of glyphosate and glufosinate in vegetables and fruits by GC-FPD Japanese

摘要

A rapid analytical method for residues of the herbicide, glyphosate N-(phosphonomethyl)glycine, glufosinate DL-homoalanine-4-yl(methyl)phosphinic acid and glufosinate metabolite (MPPA: 3-methylphosphinicopropionic acid) in vegetables and fruits was developed by improving the bulletin method of glufosinate. 50 mL of solution extracted with water (corresponding to 2 g of the sample) was loaded on a column packed with 5 mL of anion exchange resin and then the trapped glyphosate, glufosinate and MPPA were eluted with 60 mL of 50 acetic acid. After derivatization with trimethyl orthoacetate, the derivatives were purified and separated on a Florisil cartridge column. The determination of the derivatives was performed with GC-FPD. The detection limits for glyphosate, glufosinate and MPPA were 0.01 mug/g, 0.01 mug/g and 0.005 mug/g, respectively. The recoveries from fortified samples were 83.5-89.8 for glyphosate, 77.9-92.2 for glufosinate and 75.0-87.2 for MPPA.