HPLC Determination of Sugars and Starch in Green Beans

摘要

ABSTRACTA reversed‐phase HPLC method using a Spherisorb NH2column and 85:15 v/v acetonitrile/water as mobile phase was developed for determination of starch and soluble sugars (glucose, fructose and sucrose) in plants. When applied to pure starch samples, the analytical method was as accurate as the official AOAC method and had better precision (coefficient of variation 0.87 vs 1.27). When applied to green bean (Phaseolus vulgarisL.), recovery was 97.7 for starch, 98.0 for glucose and 98.1 for both fructose and sucros