Determination of Alpha‐Keto Acids in Banana Pulp by High Performance Liquid Chromatography

摘要

ABSTRACTA liquid chromatographic procedure has been developed for the separation and quantification of α‐keto acids. The DNPH‐derivatives are separated on a column of LiChrosorb Si‐60 (5 μm), eluted with petroleum ether/ethyl formate/propionic acid (65/35/5, v/v/v), and the column effluent is monitored by UV spectrophotometory at 350 nm. A total analysis of DNPH‐keto acids was completed in less than 10 min. The amount of pyruvic, α‐ketoglutaric, and oxaloacetic acid in ripe banana pulp was 347, 225, and 13 μg/100g fresh weight, respectively. This technique has been used effectively to analyze α‐keto acids in bi