Growth inhibitory and biocidal activity of some isothiazolone biocides

摘要

Collier, P.J., Ramsey, A.J., Austin, P.Gilbert, P. 1990. Growth inhibitory and biocidal activity of some isothiazolone biocides.Journal of Applied Bacteriology69, 569–577.Similar patterns of growth inhibition were observed for the three biocides, benzisothiazol‐3‐one (BIT), 5‐chloro‐N‐methylisothiazol‐3‐one (CMIT) and N‐methylisothiazol‐3‐one (MIT) againstEscherichia coliATCC 8739 andSchizo‐saccharomyces pombeNCYC 1354. After periods of induced stasis, proportional to biocide concentration, growth proceeded at an inhibited rate. Extrapolation of the static periods and inhibited growth rates against biocide concentration gave minimum growth inhibitory concentration estimates of 0.1–0.5 μg/ml for CMIT, 15–20 μg/ml for BIT and 40–250 μg/ml for MIT. Patterns of growth inhibition by CMIT and induced morphological changes in inhibited cultures suggested this com‐pound to also inhibit initiation of DNA replication. Growth inhibitory activity was rapidly quenched by the addition of thiol‐containing materials such as glutathione and cysteine. The activity of CMIT was additionally quenched by the presence of the non‐thiol amino acids valine and/or histidine. These results suggest that the chlorinated isothiazolones can react with amines