Mitochondrial Genome Diversity within a Cultivar ofDaucus carota(ssp.sativus) Revealed by Restriction Fragment Analysis of Single Plants

摘要

AbstractAn effective method for the isolation and restriction fragment analysis of mitochondrial (mt) DNA from a few grams of plant material ofDaucus carota sativusis presented. Employing radioactive end‐labelling of mtDNA restriction fragments, 12 single plants from a carrot cultivar, ‘Vitaminaja’, were investigated for mitochondrial genome diversity. Analyses revealed four different types of characteristic mtDNA restriction fragment patterns within the same cultivar. The mitochondrial genome diversity in the cultivar ‘Vitaminaja’ may result from non‐homogenous ancestor cytoplasms as well as from nuclear‐induced mtDNA rearrangements. Evaluation of intraspecific mitochondrial genome diversity may become an important issue in breedi