The development of the male gametophyte from western hemlock (Tsuga heterophylla(Raf.) Sarg.) pollen was complete after 5 days of incubation. This normally takes at least 6 weeks in vivo. The pollen was cultured in a solution containing boron, calcium nitrate, magnesium sulfate, and potassium nitrate. In 2 days the generative cell divided into the body cell and the stalk cell and after a further 3 days the body nucleus divided into two sperm nuclei. Morphological descriptions and measurements of the germinating pollen grains were made.
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