Production of polyclonal antibodies against a wheat thioredoxin h by genetic immunization.

摘要

The recent techniques of genetic immunization, in which DNA constructs are introduced directly into mammalian tissues in vivo, were used to produce antisera against thioredoxin h, a protein of wheat. Two rabbits and two mice were inoculated intramuscularly with a vector containing the cDNA encoding the protein of interest under control of a cytomegalovirus promoter. No immune response was observed in rabbits, even when a 4-fold quantity of DNA and a different inoculation site were used. In contrast, an inoculated mouse was found to produce antisera against the wheat thioredoxin h as analysed by western blotting. This technique appears useful for obtaining polyclonal antibodies against plant proteins that are difficult to purify, if the corresponding cDNAs are available.