Binding of colchicine to a soluble fraction of carrot cells grown in suspension culture

摘要

The binding of 3Hcolchicine to soluble component prepared from carrot (Daucus carotaL.) cells in suspension culture was assayed by the diethylaminoethyl(DEAE)-cellulose powder method. The binding activity was very labile and the time course of the binding indicated that the colchicine-bound complex was also unstable. The reaction was enhanced by vinblastine but lumicolchicine had no effect. The optimum temperature for the reaction was 30° C, and the colchicine binding constant was calculated to be 3.5·104l mol−1at 30