EFFECT OF ADDITIVES ON PRESERVABILITY AND FREEZABILITY OF BOVINE SEMEN (HF AND MURRAH) IN TRIS DILUTOR

摘要

Incorporation of additives cysteine HC1, EDTA, sugars, raffinose and fructose, alone or in combination with each other improved post preservation and post-thaw motility. The improvement was more pronounced for HF semen than buffalo semen. Post-thaw motility of HF semen was 58.2 ± 1.9% and 56.3 ± 2.1 for cyiteine and EDTA, respectively as compared to 52.2 ± 2.6% for control. The respective values for buffalo semen was 58.3 ± 1.3, 53.2 ± 2.2 and 50.6 ± 1.2%. Cysteinc HCl was superior than ED TA for both species. The effects of these additives were more pronounced in presence of glycerol than without glycerol. Raffinose and fructose also provided good motility during in vitra preservation. But addition of both additives and sugars altogether didnot provide much beneficial effect during preservation or freezing, however raffinose was slightly better over fructose, in combination. These additives and sugars also provided better protection during thermo-resistance tests, as past-thaw aging motiliuy and pott-thaw incubation motility improved significantly in presence of these additives.