Inhibitory Effect of Tyrosine Kinase Inhibitors on Antigen-Induced Tracheal Contraction and Histamine Release in Guinea Pigs

摘要

We examined the effect of two different types of tyrosine kinase inhibitor, herbimycin A and genistein, on antigen-induced tracheal contraction and antigen-induced histamine release from the trachea in sensitized guinea pigs in vitro. Herbimycin A (1–10 μM) and genistein (1–10 μM) significantly inhibited antigen-induced tracheal contraction, compared with control. Additionally, herbimycin A (1–10 μM) and genistein (3–10 μM) significantly inhibited antigen-induced histamine release from the trachea in a concentration-dependent manner, compared with control. On the contrary, herbimycin A and genistein did not suppress acetylcholine- and histamine-induced tracheal contraction. We concluded that herbimycin A and genistein inhibit antigen-induced tracheal contraction without inhibiting smooth muscle contractility, and these inhibitory effects are due to, at least partially, inhibiting histamine release from tracheal