Transformation of lentil (Lens culinaris M.) cotyledonary nodes by vacuum infiltration of Agrobacterium tumefaciens

摘要

Lentil cotyledonary nodes are some of the most regenerative tissues in legumes. Attempts to transform them by vacuum filtration have been Limitedly successful. This report describes a rapid and convenient transient expression protocol based on vacuum infiltration of Agrobacterium cells into lentil cotyledonary nodes. Vacuum-infiltrated tissues had significantly (P < .05) higher transient GUS expression than did noninfiltrated tissues. Under optimal conditions (infiltration at 200 mmHg for 20 min), 95 of the Agrobacterium-infiltrated explants exhibited an average of 16 blue foci. We believe this to be the first report of this technique for transient gene expression in lentil cotyledonary nodes.