Evaluation of serological tests for the diagnosis of brucellosis

摘要

Presently the incidence of abortions in bovine and ovine population in Rajasthan showed a rising trend, thus it is essential to provide suitable diagnostic test for diagnosis of brucellosis in cattle, which bears reliability, repeatability and specificity. It is planned to evaluate early developed serological tests like Rapid Plate Agglutination Test (RPAT), Serum Tube Agglutination Test (STAT), 2-Mercaptoethanol Tube Agglutination Test (2-METAT), Heat Inactivation Test (HIT) and newly developed ELISA and dot-ELISA. One hundred fourty four serum samples were collected from Kankrej breed cattle herd had history of abortion and repeat breeding from Jain Parshunath Goshala Nakoda Dist. Barmer (Raj.). The serum samples were tested for presence of Brucella abortus antibodies using RPAT and STAT (WHO, 1967), HIT and 2-METAT (Amerault et al., 1961), ELISA (Kulkarni et al., 1991) and dot-ELISA (Hawkes, 1986). The performance of the serological tests was evaluated individually as well as compared with standard test ELISA for their relative concordance, relative sensitivity, relative specificity and relative T/N ratio as per Ruppanner et al., (1980).