A Note on the Development of a Technique for Regeneration of a Degenerated Culture

摘要

In a heterogeneous degenerated culture the method ofin situscreening of established agar colonies (the number screened being limited) cannot reveal the presence of an antibiotic‐producing organism even after mutagenic treatment, whereas the close‐range parallel streak method can succeed. Streaking a pour‐plate culture of an antibiotic‐sensitive organism by parallel streaks of mutagen‐treated inactive culture of the once producer afforded a better chance for regeneration of a degenerate