Overexpression ofc-Ki-rasandc-fosin human pancreatic carcinomas

摘要

The mRNA expression of protooncogenesc-Ki-ras, c-myc, andc-foswas studied in five pancreatic carcinomas and five normal pancreatic tissues using RNase protection assay and Northern blot analysis. The expression of those protooncogenes was detected in total mRNA from all specimens. However, the amounts in carcinomas and in normal tissues differed.C-Ki-rasmRNA in all the tumors was expressed up to sixfold more than in normal tissues.C-fosmRNA was also overexpressed up to tenfold in four of five tumors. In contrast,c-mycmRNA levels were varied and did not differ significantly between tumors and normal tissues. The results suggest that the overexpression ofc-Ki-rasandc-fosmRNA are implicated in the development of pancreatic adenocarcinoma.