A simple and efficient In vitro regeneration protocol for Bougainvillea spectabilis Willd., was developed from shoot tips of 5 year old plants. Shoot tips were surface sterilized and cultured on MS medium supplemented with different concentrations ofBAP (0.25 - 2.0mg/I) or Kinetin (0.25 - 2.0mg/l) and NAA (0.1-0.5 mg/1) in combination with BAP (0.25 - 5.0 mg/1). It was observed that BAP (0.25mg/l) combined with 0.1mg/1 NAA gave best results where 90 shoots were developed into plantlets. Best multiple shoot formation was recorded on MS medium supplemented with 1.0mg/1 BAP and 250 mg/ 1 glutamine. The regenerated shoots were successfully rooted on half strength MS medium with different concentrations and combinations of auxin. It was observed that 2.5 mg/1 NAA combined with 2.5mg/l IBA gave 100 root induction. The plantlets after weaning and acclimatization were transferred to pots and then to soil for establishment.
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