A modern method of performing conventional countercurrent distribution called Dual Countercurrent Chromatography is described. The system consists of a multilayer coiled column integrated with two inlet and two outlet lines for solvent phases and a sample feed line. Subjecting the system to a particular combination of centrifugal and planetary motions produces a unique hydrodynamic effect which allows two immiscible liquids to flow countercurrently through the coiled column. The sample solution is fed at the middle portion of the column and eluted simultaneously through the column in the opposite direction by two liquids. This distinct feature of maintaining a constant fresh two phases within the coiled column permits a rich domain of application, such as continuous sample process and continuous extraction.
展开▼
